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SCI醫(yī)學(xué)論文翻譯

SCI醫(yī)學(xué)論文翻譯是醫(yī)學(xué)科學(xué)研究工作的書面記錄與文字總結(jié)得出來的論文,是醫(yī)學(xué)科學(xué)研究工作無法缺少的重要部份, 是目前學(xué)歷、學(xué)位、晉升職稱的必需條件。因此,寫出一個涵義準(zhǔn)確、清楚、文字精簡且不乏味的英文SCI醫(yī)學(xué)論文翻譯是件較有挑戰(zhàn)性的事情,譯員需要有相較不錯的英語寫作能力跟醫(yī)學(xué)背景, SCI醫(yī)學(xué)論文翻譯基本要求是:清楚、準(zhǔn)確、簡潔和生動。

SCI醫(yī)學(xué)論文翻譯案例:

Gene therapy was introduced in treatment of Hepatitis C since molecular biological techniques have developed, and the pathogenesis of Hepatitis C has been gradually made clear. To choose a highly-effective, specific, and safe hepatic-targeting gene vector is the key to the success of genetic treatment of hepatic disease. Recently, cationic polymer is extensively applied for its stability, being easy to modify, and low immunogenicity, especially the polyvalent cationic polymer polyethyleneimine (PEI), which can be degraded in physiological environment [1-3]. Chitosan (CS), a natural polycation gene vector has attracted extensive attention [4,5].  In PET/CS induced hepatic-targeting gene introduction, actively recognizing and endocytosing the transfection compound containing its specific ligand through asialoglycoprotein receptor (ASGP-R) is the major mechanism of improving the efficiency of the gene transference. Also it is a receptor-mediated gene transference system which is widely studied at present.

In this research we grafted the low-molecular-weight PEI (2K) to galactosylated chitosan following the documents [6], and synthesized galactosylated chitosan- polyethyleneimine (2K) (GC-PEI), studies the targeting of GC-PEI/DNA complex in hepatic cell lines (L02, QSG7701 and QSG7701/core) and hepatic cell in mouse. To optimize the transfection efficiency of gene vectors, we synthesized GC-PEI/DNA complexes in three different solvents, and selected appropriate gene vectors for experiment in vitro and in vivo.  

Materials and Methods

Main reagents and equipments

Reagents included Branched Polyethylenimine (M.W 25KD, no water, PEI25K), Branched Polyethylenimine (M.W 2000, 50% water, PEI (2K)), N-Hydroxysuccinimide (NHS), 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), Lactobionic Acid (LA), 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), potassium periodate (purchased from Sigma –Aldrich Corporation), chitosan (from Shanghai Bio Science and Technology Co., Ltd., deacetylation degree 93%), DNA PolymerasesⅠ (from Roche Corporation in Switzerland), DMEM medium (from Gibco Corporation), and plasmid pEGFP-C1 (from Clontech Corporation). The reagents except for the annotated ones were of analytical pure.

Equipment included dialysis bag of 12000-14000KDa, dialysis bag of 3500Da purchased from Pierce Corporation in the USA, transmission electron microscope from Simens in Germany, freezer dryer from Thremo Corporation in Germany, and particle size and potential analyzer from Malvern Corporation in England.

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